技术文章
Technical articles产品名称 THLE-2(人肝永生化细胞)
别称 THLE2;Transformed Human Liver Epithelial-2
种属 人
生长特性 贴壁细胞
细胞形态 上皮细胞样
冻存条件 冻存液:55% DM/F12+40% FBS+5% DMSO
温度:液氮
培养方案A(默认) 生长培养基:THLE-2细胞专用培养基
培养条件:气相:空气,95%;CO₂,5%, 温度:37℃
推荐传代比例 1:3-1:6
推荐换液频率 2-3次/周
参考资料(来源文献)
背景描述 THLE-2 is an epithelial cell that was isolated from the left lobe of a donor.THLE-2 and THLE-3 cells express phenotypic characteristics of normal adult liver epithelial cells. They are nontumorigenic when injected into athymic nude mice, have near-diploid karyotypes, and do not express alpha-fetoprotein. [RF84750] THLE-2 and THLE-3 cells metabolize benzo[a]pyrene, N-nitrosodimethylamine, and aflatoxin B1 to their ultimate carcinogenic metabolites that adduct DNA, which indicates functional cytochrome P450 pathways.
年龄(性别) Adult;Male
组织来源 Liver; Left lobe
细胞类型 转化细胞系
生物安全等级 BSL-2
致瘤性 No; No, nude mice.
保藏机构 ATCC; CRL-2706