THLE-2（人肝永生化细胞）说明书

产品名称    THLE-2（人肝永生化细胞）

别称    THLE2;Transformed Human Liver Epithelial-2

种属    人

生长特性    贴壁细胞

细胞形态    上皮细胞样

冻存条件    冻存液：55% DM/F12+40% FBS+5% DMSO

温度：液氮

培养方案A（默认）    生长培养基：THLE-2细胞专用培养基

培养条件：气相：空气，95%；CO₂，5%, 温度：37℃

推荐传代比例    1:3-1:6

推荐换液频率    2-3次/周

参考资料(来源文献)

背景描述    THLE-2 is an epithelial cell that was isolated from the left lobe of a donor.THLE-2 and THLE-3 cells express phenotypic characteristics of normal adult liver epithelial cells. They are nontumorigenic when injected into athymic nude mice, have near-diploid karyotypes, and do not express alpha-fetoprotein. [RF84750] THLE-2 and THLE-3 cells metabolize benzo[a]pyrene, N-nitrosodimethylamine, and aflatoxin B1 to their ultimate carcinogenic metabolites that adduct DNA, which indicates functional cytochrome P450 pathways.

年龄（性别）    Adult;Male

组织来源    Liver; Left lobe

细胞类型    转化细胞系

生物安全等级    BSL-2

致瘤性    No; No, nude mice.

保藏机构    ATCC; CRL-2706